human lung fibroblast wi38 cell line  (ATCC)

Journal: BMC Complementary Medicine and Therapies

Article Title: Marine actinobacterium Streptomyces vinaceusdrappus mediated nano-selenium: biosynthesis and biomedical activities

doi: 10.1186/s12906-025-05073-9

Figure Lengend Snippet: Cell viability of Wi38, Caco-2, and PANC-1 cells treated with biogenic Se-NPs (31.2–1000 µg mL –1 ). Different letters ( a , b , and c ) on the bars at the same concentration indicate the results are significantly different ( n = 3, P ≤ 0.05)

Article Snippet: To assess the cytotoxic activity of Se-NPs using crystal violet viability assay, three different cell lines were investigated: the WI38 human fibroblast cell line (ATCC CCL-75), the Caco-2 human colon adenocarcinoma cell line (ATCC HTB-37), and the PANC-1 human pancreatic cancer cell line (ATCC CRL-1469).

Techniques: Concentration Assay

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding approaches for tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds: (A) Wi38 monoculture, (B) Calu-3 monoculture, and (C) coculture.

Article Snippet: Wi38 cell line (CCL-75, ATCC).

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding of the OL of tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds using Wi38 cells. (A) Cell metabolic activity of Wi38 cells on the OL and IL on days 1, 5, and 7. (B) DNA quantification on the OL and IL at day 7. (C) Coverage of the IL by Wi38 cells at day 7. Representative images of Wi38 cells growing on the OL using three seeding densities: (D) 1.5 × 10 5 cells/cm 2 , (E) 3 × 10 5 cells/cm 2 and (F) 6 × 10 5 cells/cm 2 . Cells were treated with DAPI (blue) and phalloidin (red) to stain the nuclei and actin filaments, respectively. Images were captured via confocal microscopy (Axio Examiner.Z1 confocal microscope). Results displayed as mean ± SEM ( n = 3, * p < 0.05, ** p < 0.01).

Article Snippet: Wi38 cell line (CCL-75, ATCC).

Techniques: Activity Assay, Staining, Confocal Microscopy, Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic acid (t-PCL-CHyA-B) scaffolds seeded on the outer layer (OL) with Wi38 cells and the inner layer (IL) with Calu-3 cells for 4h under rotation using 3D printed accessories. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds seeded with (C) Wi38 monoculture, (D) Calu-3 monoculture and (E) coculture using 3D printed accessories. Images were obtained using a Axio Examiner. Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3, ** p < 0.01, **** p < 0.0001).

Article Snippet: Wi38 cell line (CCL-75, ATCC).

Techniques: Activity Assay, Microscopy, Staining

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds seeding the OL with Wi38 cells and the IL with Calu-3 cells for 2 h under rotation. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds: (D) Wi38 monoculture, (E) Calu-3 monoculture, and (F) coculture. Images were obtained using an Axio Examiner Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3).

Article Snippet: Wi38 cell line (CCL-75, ATCC).

Techniques: Activity Assay, Microscopy, Staining

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding approaches for tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds: (A) Wi38 monoculture, (B) Calu-3 monoculture, and (C) coculture.

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Seeding of the OL of tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds using Wi38 cells. (A) Cell metabolic activity of Wi38 cells on the OL and IL on days 1, 5, and 7. (B) DNA quantification on the OL and IL at day 7. (C) Coverage of the IL by Wi38 cells at day 7. Representative images of Wi38 cells growing on the OL using three seeding densities: (D) 1.5 × 10 5 cells/cm 2 , (E) 3 × 10 5 cells/cm 2 and (F) 6 × 10 5 cells/cm 2 . Cells were treated with DAPI (blue) and phalloidin (red) to stain the nuclei and actin filaments, respectively. Images were captured via confocal microscopy (Axio Examiner.Z1 confocal microscope). Results displayed as mean ± SEM ( n = 3, * p < 0.05, ** p < 0.01).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Staining, Confocal Microscopy, Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic acid (t-PCL-CHyA-B) scaffolds seeded on the outer layer (OL) with Wi38 cells and the inner layer (IL) with Calu-3 cells for 4h under rotation using 3D printed accessories. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds seeded with (C) Wi38 monoculture, (D) Calu-3 monoculture and (E) coculture using 3D printed accessories. Images were obtained using a Axio Examiner. Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3, ** p < 0.01, **** p < 0.0001).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining

Journal: ACS Biomaterials Science & Engineering

Article Title: Method for Targeted Cellular Seeding of Tubular Tissue-Engineered Scaffolds for Tracheal Regeneration Approaches

doi: 10.1021/acsbiomaterials.5c00365

Figure Lengend Snippet: Coculture on tubular bilayered PCL-Collagen and Hyaluronic (t-PCL-CHyA-B) scaffolds seeding the OL with Wi38 cells and the IL with Calu-3 cells for 2 h under rotation. (A) Cell metabolic activity at days 2, 7, and 10 of culture. (B) Coverage of the IL at day 10 of culture. Representative images of the IL of t-PCL-CHyA-B scaffolds: (D) Wi38 monoculture, (E) Calu-3 monoculture, and (F) coculture. Images were obtained using an Axio Examiner Z1 confocal microscope. Cells were stained with DAPI (blue) to label nuclei and phalloidin (red) to stain actin filaments. Results displayed as mean ± SEM ( n = 3).

Article Snippet: The Wi38 human embryonic lung fibroblast cell line (ATCC) was cultured in T175 tissue culture flasks following revival.

Techniques: Activity Assay, Microscopy, Staining

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Cytotoxic effect of doxorubicin concentrations used for senescence induction on WI38 and A549. ( A ) the percentage of viable cells with ( B ) representative images of live (no fluorescent) and dead (fluorescent, orange) cells for both cell lines. ns— p > 0.05, *– p ≤ 0.05, **– p ≤ 0.01, ****— p ≤ 0.0001.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Senescent features of WI38 cell line. ( A ) percentage of SA-β-gal-positive and negative cells with ( B ) representative images of stained cells for control and selected concentrations of doxorubicin used for cellular senescence induction. SA-β-gal-positive cells are pointed out with black arrows (Full size images are in ). ( C ) percentage of cells actively proliferative and ( D ) representative images of nuclei of all cells in population stained with Hoechst 33342 (blue); proliferative cells were stained with EdU (red) and both images merged together. ****— p ≤ 0.0001.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Morphological and nuclear features of senescent WI38 cells. ( A ) Representative fluorescent images showing the morphology of senescent WI38 cells in comparison to untreated control cells. Actin filaments were stained with phalloidin-488 (green), and cell nuclei were counterstained with Hoechst 33342 (blue). The final panel presents a merged view of both channels. Scale bar: 50 μm. ( B ) Hoechst 33342 staining reveals the presence of SAHF, represented by lighter dots on the nuclei structure (the image from yellow box is presented on the right to help visualize SAHF).

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Comparison, Control, Staining

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Histograms showing size differences between senescent and untreated ( A ) WI38 and ( B ) A549 cells, measured by imaging flow cytometry. Representative brightfield images of analyzed cells are shown below the corresponding histograms.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Imaging, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: sasp analysis of IL-6 and IL-8 secretion in ( A ) WI38 and ( B ) A549 cells before and after senescence induction. s—senescent, ns— p > 0.05, **— p ≤ 0.01, and ****— p ≤ 0.0001.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Cellular uptake of Nile red-labeled liposomes. Confocal 3D image showing ( A ) WI38 and ( B ) A549 cells stained with phalloidin-488 (green) to visualize actin filaments and Hoechst 33342 (blue) to label nuclei. Liposomes loaded with Nile red (red). White arrows indicate liposomes internalized by the cell. Scale bar: 10 μm.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Labeling, Liposomes, Staining

Journal: International Journal of Molecular Sciences

Article Title: Targeting Cellular Senescence with Liposome-Encapsulated Fisetin: Evidence of Senomorphic Effect

doi: 10.3390/ijms26157489

Figure Lengend Snippet: Interleukin concentrations following senotherapy, measured by ELISA. ( A ) IL-6 and ( B ) IL-8 levels in WI38 cells. n-sen—non-senescent, sen—senescent. ns— p > 0.05, *— p ≤ 0.05, **— p ≤ 0.01, ***— p ≤ 0.001, and ****— p ≤ 0.0001.

Article Snippet: A549 and WI38 cell lines were purchased from ATCC (Manassas, VA, USA) and maintained in EMEM enriched with 10% FBS, 1% sodium pyruvate, and 1% antibiotics (penicillin 100 μg/mL, streptomycin 100 μg/mL) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Enzyme-linked Immunosorbent Assay